Descrição:
New. New Book; Fast Shipping from UK; Not signed; Not First Edition; The E. coli Plasmid Vectors : Methods and Applications.
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E. Coli Plasmid Vectors: Methods and Applications Brochura - 2010
por Nicola Casali (Editor); Andrew Preston (Editor)
Descrição da contracapa
Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results.
Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.
Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.
Detalhes
- Título E. Coli Plasmid Vectors: Methods and Applications
- Autor Nicola Casali (Editor); Andrew Preston (Editor)
- Encadernação Brochura
- Edição Softcover reprin
- Páginas 316
- Volumes 1
- Idioma ENG
- Editorial Humana
- Data de publicação 2010-11-10
- ISBN 9781617373916 / 1617373915
- Peso 1.07 libras (0.49 kg)
- Dimensão 9 x 6 x 0.74 in. (22.86 x 15.24 x 1.88 cm)
- Dewey Decimal Code 572.869
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E. coli Plasmid Vectors : Methods and Applications
por Nicola Casali
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- 9781617373916 / 1617373915
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Uxbridge, Greater London, United Kingdom
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E. coli Plasmid Vectors
- Novo
- Condição
- Novo
- ISBN 10 / ISBN 13
- 9781617373916 / 1617373915
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- 4
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Woodside, New York, United States
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€ 158,77€ 3,77 frete para USA
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Descrição:
Humana Press , pp. 330 . Papeback. New.
Preço do item
€ 158,77
€ 3,77
frete para USA